Chinese Journal of Catalysis

Chinese Journal of Catalysis ›› 2006, Vol. 27 ›› Issue (10): 875-879.

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Synthesis and Catalytic Kinetics of Tellurium-Modified Hyaluronic Acid with Glutathione Peroxidase Activity

ZHANG Boxun1, CHEN Zhibo1, AN Yang3, PENG Qinglin1, CHEN Jia1, ZHANG Jianguo2, LIU Lanying1*   

  1. 1 College of Life Science, Jilin University, Changchun 130023, Jilin, China; 2 State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, The Chinese Academy of Sciences, Changchun 130022, Jilin, China; 3 Hospital of Jilin University, Changchun 130021, Jilin, China
  • Received:2006-10-25 Online:2006-10-25 Published:2010-11-28

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Abstract: A novel mimic TeHA was synthesized by modifying hyaluronic acid (HA) with tellurium, whose function is similar to that of glutathione peroxidase (GPX). The structure of TeHA was characterized by means of infrared spectroscopy and nuclear magnetic resonance spectroscopy, showing that the target Te is located at -CH2OH of the N-acetyl-D-glucosamine of HA. The activity of TeHA is 163.6 U/μmol according to Wilson′s method. In contrast to other mimics, TeHA displays a high activity. Moreover, TeHA can use many hydroperoxides as substrates, such as H2O2, cumenyl hydroperoxide, and tert-butyl hydroperoxide, and cumenyl hydroperoxide is the optimal substrate. A ping-pong mechanism was deduced for the reduction reactions catalyzed by TeHA according to the steady-state kinetic studies.

Key words: hyaluronic acid, tellurium, glutathione peroxidase, kinetics