Immobilization of Bacillus megaterium Epoxide Hydrolase and Its Catalytic Performance

Chinese Journal of Catalysis ›› 2008, Vol. 29 ›› Issue (1): 47-51.

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Immobilization of Bacillus megaterium Epoxide Hydrolase and Its Catalytic Performance

JIA Tao1, XU Jianhe1*, YANG Sheng2

1 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; 2 Shanghai Institute of Plant Physiology and Ecology, The Chinese Academy of Sciences, Shanghai 200232, China

Received:2008-01-25 Online:2008-01-25 Published:2011-12-25

Abstract

Abstract: The immobilization of Bacillus megaterium ECU1001 epoxide hydrolase was investigated. Macro-reticular DEAE-cellulose was selected to immobilize the epoxide hydrolase and the activity retention was about 70%. The effect of temperature and pH on the immobilized epoxide hydrolase was measured. Batch reactions of the hydrolysis of rac-glycidyl phenyl ether were conducted with the immobilized epoxide hydrolase at a low substrate concentration. The residual enzyme activity of 72.4% was achieved after 10 cycles of reaction.

Key words: Bacillus megaterium, epoxide hydrolase, immobilization, glycidyl phenyl ether, hydrolysis

JIA Tao;XU Jianhe*;YANG Sheng. Immobilization of Bacillus megaterium Epoxide Hydrolase and Its Catalytic Performance[J]. Chinese Journal of Catalysis, 2008, 29(1): 47-51.

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Immobilization of Bacillus megaterium Epoxide Hydrolase and Its Catalytic Performance

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