Chinese Journal of Catalysis ›› 2009, Vol. 30 ›› Issue (1): 48-52.

• Articles • Previous Articles     Next Articles

Enhancement of Arctigenin Content by Processing of Fructus Arctii via Solid-State Fermentation Coupled with Poach

OU Zhimin1, GUO Fangyuan1, YANG Gensheng1*, YAO Shanjing2   

  1. 1 Pharmaceuticals College, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China; 2 Institute of Bioengineering, Zhejiang University, Hangzhou 310027, Zhejiang, China
  • Received:2009-01-25 Online:2009-01-25 Published:2012-10-17

Abstract: The arctigenin content in fructus arctii has been increased by solid-state ferm entation coupled with poach. The optimum medium components are 0.5 g fructus ar c tii powder, 3 g bran, 2 g bagasse, 0.3 g peptone, and 10 ml Mandels nutrient fl uid. Solid-to-liquid ratio is 1∶3.6 g/ml. Initial pH of the medium is 5.6. The yield reached 93.0% when 0.174 mmol/L arctiin was hydrolyzed at 30 ℃ for 7 d. In 7 d, the hydrolysis of arctiin is in accordance with the equation of Monod.

Key words: arctiin, arctigenin, β-glucosidase, Chinese medicine, fermentation, Aspergillus niger ZJUT712