Biotransformation of geniposide in Gardenia jasminoides to genipin by Trichoderma harzianum CGMCC 2979

doi:10.1016/S1872-2067(14)60134-0

Chinese Journal of Catalysis ›› 2014, Vol. 35 ›› Issue (9): 1534-1546.DOI: 10.1016/S1872-2067(14)60134-0

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Biotransformation of geniposide in Gardenia jasminoides to genipin by Trichoderma harzianum CGMCC 2979

Yuesheng Dong^a, Leping Liu^a, Yongming Bao^a, Aiyu Hao^b, Ying Qin^a, Zujia Wen^a, Zhilong Xiu^a

a School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, Liaoning, China;
b Dalian Institute for Drug Control, Dalian 116021, Liaoning, China

Received:2014-02-14 Revised:2014-04-04 Online:2014-08-19 Published:2014-08-22
Supported by:
This work was Supported by the National Natural Science Foundation of China (81172966), the National Basic Research Program of China (973 Program, 2009CB724706), and the Fundamental Research Funds for the Central Universities (DUT12ZD209).

Abstract

Abstract:

Trichoderma harzianum (T. harzianum) CGMCC 2979 was used to transform the geniposide in Gardenia jasminoides (G. jasminoides) to genipin, dispensing the use of purified enzyme and the extraction of geniposide from the raw material. At 30 ℃, pH 6.1, and an initial G. jasminoides concentration of 80 g dried fruit per liter of medium, the geniposide-to-genipin conversion rate reached 97.8% after 48 h of fermentation. The genipin was purified from the fermentation broth by a combined method of XAD-16N-resin and silica-gel chromatography, yielding a total recovery of 62.3%. A 74.4-kDa geniposide-β-glucosidase implicated in the transformation of geniposide to genipin was purified from T. harzianum CGMCC 2979. It had optimum activity at 50 ℃ and pH 4.0-5.0. The K_m and V_max of the enzyme for geniposide were 3.6 mmol/L and 775 μmol/h/mg protein, respectively. The simple, direct, and efficient biotransformation of geniposide in G. jasminoide to genipin by T. harzianum CGMCC 2979 that is described in this study could represent an alternative and effective method for producing genipin.

Key words: Geniposide, Genipin, Trichoderma harzianum, Biotransformation, Geniposide-β-glucosidase, Purification

Yuesheng Dong, Leping Liu, Yongming Bao, Aiyu Hao, Ying Qin, Zujia Wen, Zhilong Xiu. Biotransformation of geniposide in Gardenia jasminoides to genipin by Trichoderma harzianum CGMCC 2979[J]. Chinese Journal of Catalysis, 2014, 35(9): 1534-1546.

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Biotransformation of geniposide in Gardenia jasminoides to genipin by Trichoderma harzianum CGMCC 2979

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