Immobilization of cellulase onto MnO2 nanoparticles for bioethanol production by enhanced hydrolysis of agricultural waste

doi:10.1016/S1872-2067(15)60906-8

Abstract

Abstract:

Cellulase is an efficient enzymatic catalyst that hydrolyses cellulosic substances. The high costs associated with using enzymes for industrial applications can be reduced by immobilizing the cellulase. In the current study, cellulase produced by Aspergillus fumigatus JCF was immobilized onto MnO₂ nanoparticles, which improve the activity of cellulase and offer a superior support. The surface characteristics of synthesized MnO₂ nanoparticles and cellulase-bound MnO₂ nanoparticles were investigated by scanning electron microscopy, and Fourier transform infrared spectroscopy was used to analyze the functional characteristics of the immobilized cellulase. The maximum cellulase binding efficiency was 75%. The properties of the immobilized cellulase, including activity, operational pH, temperature, thermal stability, and reusability were investigated and were found to be more stable than for the free enzyme. It was found that cellulase immobilized on MnO₂ nanoparticles could be used to hydrolyze cellulosic substances over a broad range of temperature and pH. The results confirmed that cellulase immobilized on MnO₂ nanoparticles was very efficient in terms of cellulolytic activity.

Key words: Cellulase, Immobilization, Manganese dioxide, Nanobiocatalyst, Agricultural waste, Hydrolysis, Bioethanol

Elsa Cherian, Mahendradas Dharmendirakumar, Gurunathan Baskar. Immobilization of cellulase onto MnO₂ nanoparticles for bioethanol production by enhanced hydrolysis of agricultural waste[J]. Chinese Journal of Catalysis, 2015, 36(8): 1223-1229.

×
share this article

Add to citation manager EndNote|Ris|BibTeX

URL: https://www.cjcatal.com/EN/10.1016/S1872-2067(15)60906-8

https://www.cjcatal.com/EN/Y2015/V36/I8/1223

References

[1] Olofsson K, Wiman M, Liden G. J Biotechnol, 2010, 145: 168
[2] Jagtap S, Rao M. Biochem Biophys Res Commun, 2005, 329: 111
[3] Guo R, Ding M, Zhang S L, Xu G L, Zhao F K. J Comp Physiol B, 2008, 178: 209
[4] Walsh G. In: Proteins: Biochemistry and Biotechnology. New York: Wiley, 2001
[5] Liao H D, Chen D, Yuan L, Zheng M, Zhu Y H, Liu X M. Carbohyd Polym, 2010, 82: 600
[6] Vaillant F, Millan A, Millan P, Dornier M, Decloux M, Reynes M. Process Biochem, 2000, 35: 989
[7] Mao X P, Guo G J, Huang J F, Du Z Y, Huang Z S, Ma L, Li P, Gu L Q. J Chem Technol Biotechnol, 2006, 81: 189
[8] Wu L L, Yuan X Y, Sheng J. J Membr Sci, 2005, 250: 167
[9] Gupta A K, Gupta M. Biomaterials, 2005, 26: 3995
[10] Annamalai N, Rajeswari M V, Elayaraja S, Balasubramanian T. Carbohyd Polym, 2013, 94: 409
[11] Klostergaard J, Seeney C E. Maturitas, 2012, 73: 33
[12] Sung Y J, Suk H J, Sung H Y, Li T H, Poo H, Kim M G. Biosens Bioelectron, 2013, 43: 432
[13] Savage N, Diallo M S. J Nanopart Res, 2005, 7: 331
[14] Anirudhan T S, Rauf T A. Colloids Surf B, 2013, 107: 1
[15] Tratnyek P G, Johnson R L. Nano Today, 2006, 1: 44
[16] Ansari S A, Husain Q. Biotechnol Adv, 2012, 30: 512
[17] Gupta M N, Kaloti M, Kapoor M, Solanki K. Artif Cells Blood Substit Immobil Biotechnol, 2011, 39: 98
[18] Zhang Y C, Qiao T, Hu X Y. J Solid State Chem, 2004, 177: 4093
[19] Gopalakrishnan I K, Bagkar N, Ganguly R, Kulshreshtha S K. J Cryst Growth, 2005, 280: 436
[20] He X, Wang Z H, Geng D Y, Zhang Z D. J Mater Sci Technol, 2011, 27: 503
[21] Mei X Y, Liu R H, Shen F, Wu H J. Energy Fuels, 2009, 23: 487
[22] Han M, Kim Y, Kim Y, Chung B, Choi G W. Korean J Chem Eng, 2011, 28: 119
[23] Harish Kuma M, Poonam S. Int J Chem Chem Eng, 2013, 3: 155
[24] Khattak W A, Ul-Islam M, Park J K. Korean J Chem Eng, 2012, 29: 1467
[25] Verma M L, Chaudhary R, Tsuzuki T, Barrow C J, Puri M. Bioresour Technol, 2013, 135: 2
[26] Abraham R E, Verma M L, Barrow C J, Puri M. Biotechnol Biofuels, 2014, 7: 90
[27] Lineweaver H, Burk D. J Am Chem Soc, 1934, 56: 658
[28] Miller G L. Anal Chem, 1959, 31: 426
[29] Balasubramanian K, Ambikapathy V, Panneerselvam A. J Microbiol Biotechnol Res, 2011, 1(4): 158
[30] Arica M Y, Bayramoglu G, Bicak N. Process Biochem, 2004, 39: 2007
[31] Ahmed R, Sardar M. Indian J Biochem Biophys, 2014, 51: 314
[32] Goldstein L, Levin Y, Katchalski E. Biochemistry, 1964, 3: 1913
[33] Gokhale A A, Lu J, Lee I. J Mol Catal B, 2013, 90: 76
[34] Jordan J, Kumar C S S R, Theegala C. J Mol Catal B, 2011, 68: 139
[35] Cao M, Li Z H, Wang J L, Ge W P, Yue T L, Li R H, Colvin V L, Yu W W. Trends Food Sci Technol, 2012, 27: 47
[36] Bellamy L J. The infrared Spectra of Complex Molecules. 3rd ed. New York: John Wiley, 1975
[37] Silverstein R M, Webster F X. Spectrometric Identification of Organic Compounds. 6th ed. New York: John Wiley, 1998
[38] Jones R P, Pamment N, Greenfield P F. Process Biochem, 1981, 16: 42
[39] Takagi M, Abe S, Suzuki S, Emert G H, Yata N. In: Ghose T K ed. Proceedings of Bioconversion of Cellulosic Substances into Energy, Chemicals and Microbial Protein. Delhi: Indian Institute of Technology Delhi, 1977. 551
[40] Zhang Z S, Donaldson A A, Ma X X. Biotechnol Adv, 2012, 30: 913
[41] Wei W, Yuan T Q, Wang K, Cui B K, Dai Y C. Process Biochem, 2012, 47: 2552
[42] McKendry P. Bioresour Technol, 2002, 83: 37

Immobilization of cellulase onto MnO₂ nanoparticles for bioethanol production by enhanced hydrolysis of agricultural waste

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

[1]	Zhou Ren, Yang Liu, Yuan Lyu, Xiangen Song, Changyong Zheng, Zheng Jiang, Yunjie Ding. Quaternary phosphonium polymer-supported dual-ionically bound [Rh(CO)I₃]^2- catalyst for heterogeneous ethanol carbonylation [J]. Chinese Journal of Catalysis, 2021, 42(4): 606-617.
[2]	Bihua Chen, Tong Ding, Xi Deng, Xin Wang, Dawei Zhang, Sanguan Ma, Yongya Zhang, Bing Ni, Guohua Gao. Honeycomb-structured solid acid catalysts fabricated via the swelling-induced self-assembly of acidic poly(ionic liquid)s for highly efficient hydrolysis reactions [J]. Chinese Journal of Catalysis, 2021, 42(2): 297-309.
[3]	Xing Chen, Lu Yang, Wangcheng Zhan, Li Wang, Yun Guo, Yunsong Wang, Guanzhong Lu, Yanglong Guo. Immobilization of penicillin G acylase on paramagnetic polymer microspheres with epoxy groups [J]. Chinese Journal of Catalysis, 2018, 39(1): 47-53.
[4]	Lijuan Shen, Gaojie Wang, Xiaoxiao Zheng, Yanning Cao, Yufeng Guo, Ke Lin, Lilong Jiang. Tuning the growth of Cu-MOFs for efficient catalytic hydrolysis of carbonyl sulfide [J]. Chinese Journal of Catalysis, 2017, 38(8): 1373-1381.
[5]	Asep Bayu, Surachai Karnjanakom, Katsuki Kusakabe, Abuliti Abudula, Guoqing Guan. Preparation of Sn-β-zeolite via immobilization of Sn/choline chloride complex for glucose-fructose isomerization reaction [J]. Chinese Journal of Catalysis, 2017, 38(3): 426-433.
[6]	Yilong Wang, Yun Zhang, Aijun Sun, Yunfeng Hu . Characterization of a novel marine microbial esterase and its use to make D-methyl lactate [J]. Chinese Journal of Catalysis, 2016, 37(8): 1396-1402.
[7]	Qikun Zhang, Junqing Kang, Bing Yang, Leizhen Zhao, Zhaosheng Hou, Bo Tang. Immobilized cellulase on Fe₃O₄ nanoparticles as a magnetically recoverable biocatalyst for the decomposition of corncob [J]. Chinese Journal of Catalysis, 2016, 37(3): 389-397.
[8]	Dun Deng, Yun Zhang, Aijun Sun, Yunfeng Hu. Enantio-selective preparation of (S)-1-phenylethanol by a novel marine GDSL lipase MT6 with reverse stereo-selectivity [J]. Chinese Journal of Catalysis, 2016, 37(11): 1966-1974.
[9]	Wenting Luo, Yuan Lyu, Leifeng Gong, Hong Du, Miao Jiang, Yunjie Ding. Alcohol-treated SiO₂ as the support of Ir-Re/SiO₂ catalysts for glycerol hydrogenolysis [J]. Chinese Journal of Catalysis, 2016, 37(11): 2009-2017.
[10]	Shilin Cao, Pei Xu, Yongzheng Ma, Xiaoxiao Yao, Yuan Yao, Minhua Zong, Xuehui Li, Wenyong Lou. Recent advances in immobilized enzymes on nanocarriers [J]. Chinese Journal of Catalysis, 2016, 37(11): 1814-1823.
[11]	Kexing Zeng, Zhipeng Huang, Jie Yang, Yanlong Gu. Silica-supported policresulen as a solid acid catalyst for organic reactions [J]. Chinese Journal of Catalysis, 2015, 36(9): 1606-1613.
[12]	Baojiao Gao, Liqin Zhang, Tao Chen. TEMPO immobilized on polymer microspheres-catalyzed oxidation of cyclohexanol by molecular oxygen [J]. Chinese Journal of Catalysis, 2015, 36(8): 1230-1236.
[13]	Fu Yang, Shuying Gao, Cuirong Xiong, Haiqing Wang, Jin Chen, Yan Kong. Coordination of manganese porphyrins on amino-functionalized MCM-41 for heterogeneous catalysis of naphthalene hydroxylation [J]. Chinese Journal of Catalysis, 2015, 36(7): 1035-1041.
[14]	Cheng Fan, Qiang Zhang, Fei Wei. Controllable oxidation for oil recovery: Low temperature oxidative decomposition of heavy oil on a MnO₂ catalyst [J]. Chinese Journal of Catalysis, 2015, 36(2): 153-159.
[15]	Likun Zhou, Zhenlei Li, Jifeng Pang, Mingyuan Zheng, Aiqin Wang, Tao Zhang. Catalytic conversion of Jerusalem artichoke tuber into hexitols using the bifunctional catalyst Ru/(AC-SO₃H) [J]. Chinese Journal of Catalysis, 2015, 36(10): 1694-1700.