催化学报

催化学报 ›› 2006, Vol. 27 ›› Issue (4): 297-298.

• 研究快讯 • 上一篇    下一篇

睾丸酮丛毛单胞菌生物催化合成对苯二甲酸

王净1,2,田晶1,2, 许建和3   

  1. 1 大连轻工业学院分析中心, 辽宁大连 116034; 2 中国科学院大连化学物理研究所国家色谱中心, 辽宁大连 116023; 3 华东理工大学生物反应器工程国家重点实验室, 上海 200237
  • 收稿日期:2006-04-25 出版日期:2006-04-25 发布日期:1984-12-26

A Method for Producing Terephthalic Acid by Comamonas testosterone DSM6577

WANG Jing1,2, TIAN Jing1,2, XU Jianhe3   

  1. 1 Analysis Center, Dalian Institute of Light Industry, Dalian 116034, Liaoning, China; 2 National Chromatography Research and Analysis Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023, Liaoning, China; 3 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
  • Received:2006-04-25 Online:2006-04-25 Published:1984-12-26

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摘要： 以对甲基苯甲酸为唯一碳源筛选能够生物催化合成对苯二甲酸的菌株,发现只有睾丸酮丛毛单胞菌DSM6577可以氧化对甲基苯甲酸生成对苯二甲酸. 对该菌株的细胞生长及其催化对甲基苯甲酸转化为对苯二甲酸的过程进行了研究. 结果表明,底物在8 h内即可完全转化,产物的生成时间为14～31 h, 其中在21 h时产量最大,为34 mg/L.

关键词: 睾丸酮丛毛单胞菌, 生物催化, 对甲基苯甲酸, 对苯二甲酸

Abstract: p-Toluic acid was used as a sole source of carbon and energy to screen for a bacterium to produce terephthalic acid. Comamonas testosterone strain DSM6577 can oxidize p-toluic acid to terephthalic acid through sequential reactions catalyzed by a series of enzymes. The growth of the strain, degradation of p-toluic acid, and production of terephthalic acid were studied. p-Toluic acid can be completely transformed within 8 h, but the formation of terephthalic acid takes place during 14 to 31 h, and a maximal yield of 34 mg/L is obtained at 21 h.

Key words: Comamonas testosterone, biocatalysis, p-toluic acid, terephthalic acid