催化学报

催化学报 ›› 2006, Vol. 27 ›› Issue (4): 361-364.

• 研究论文 • 上一篇    下一篇

黄单胞菌生物催化合成α-熊果苷

刘春巧,张淑荣,张鹏   

  1. 北京化工大学生命科学与技术学院, 北京 100029
  • 收稿日期:2006-04-25 出版日期:2006-04-25 发布日期:1984-12-26

Biocatalytic Synthesis of α-Arbutin by Xanthomonas BT-112

LIU Chunqiao, ZHANG Shurong, ZHANG Peng*   

  1. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
  • Received:2006-04-25 Online:2006-04-25 Published:1984-12-26

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摘要： 利用黄单胞菌(Xanthomonas) BT-112进行发酵,生物催化合成α-熊果苷. 考察了反应温度、摇床转速和菌体对对苯二酚的最大耐受度、反应物浓度比、反应时间及糖的种类等因素对反应的影响. 结果表明,用本方法合成的产物为单一的α-熊果苷. 在温度35 ℃, 摇床转速180 r/min, 对苯二酚浓度48 mmol/L, 蔗糖与对苯二酚的摩尔比2∶1, 反应48 h的条件下,高达94.3%的对苯二酚转化为α-熊果苷.

关键词: &alpha, -熊果苷, 黄单胞菌, 发酵, 生物催化, 对苯二酚, 蔗糖, 化妆品

Abstract: α-Arbutin has been recently found to be a useful cosmetic ingredient since it has a stronger inhibitory effect on tyrosinase than β-arbutin that is widely used in cosmetics. However, α-arbutin is not a natural product and difficult to be chemically synthesized. In this paper, a bacterium of Xanthomonas BT-112 was isolated and used for the anomer-selective synthesis of α-arbutin from hydroquinone and sucrose. The effects of reaction temperature, shaking speed, hydroquinone concentration, and reactant composition were investigated. When the suspension of Xanthomonas BT-112 was incubated at 35 ℃ with 48 mmol/L hydroquinone and 96 mmol/L sucrose, only one form of hydroquinone glucoside, α-arbutin, was obtained as a product. Under these conditions, the conversion based on the amount of hydroquinone supplied reached 94.3%. The product was identified by 13C NMR and1H NMR.

Key words: α-arbutin, Xanthomonas, fermentation, biocatalysis, hydroquinone, sucrose, cosmetic