催化学报

催化学报 ›› 2006, Vol. 27 ›› Issue (6): 537-540.

• 研究论文 • 上一篇    

有机相中脂肪酶催化合成乳酸乙酯

赵天涛1,2, 高静2, 张丽杰1, 全学军1   

  1. 1 重庆工学院生物工程学院, 重庆 400050; 2 河北工业大学化工学院, 天津 300130
  • 收稿日期:2006-06-25 出版日期:2006-06-25 发布日期:2010-08-28

Ethyl Lactate Synthesis by Lipase in Organic Media

ZHAO Tiantao1,2, GAO Jing2*, ZHANG Lijie1, QUAN Xuejun1   

  1. 1 School of Bioengineering, Chongqing Institute of Technology, Chongqing 400050, China; 2 School of Chemical Engineering, Hebei University of Technology, Tianjin 300130, China
  • Received:2006-06-25 Online:2006-06-25 Published:2010-08-28

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1112

被引次数

62

摘要： 考察了在双底物抑制下脂肪酶催化合成乳酸乙酯,最佳溶剂为叔丁醇,在所选的几种脂肪酶中,固定化于大孔丙烯酸树脂的南极假丝酵母脂肪酶B (Novozym 435)的催化活性最好. 对反应条件进行了优化,当酸醇摩尔比为1∶8, 反应温度60 ℃, 酸浓度0.3 mol/L, 酶浓度45 g/mol, 摇床转速200 r/min时产率达到77%, 脂肪酶Novozym 435重复使用6次后产率仍然可达到60%.

关键词: 脂肪酶, 乳酸, 乙醇, 酯化, 乳酸乙酯, 双底物抑制

Abstract: Esterification of lactic acid and ethanol by lipase under double substrate inhibition was studied. The best solvent is tert-butyl alcohol and the best lipase is Novozym 435 (immobilized on a macroporous acrylic resin) for this reaction system. The optimization of reaction conditions was studied in detail. When the molar ratio of acid to alcohol is 1∶8, the temperature is 60 ℃, the concentration of acid and lipase is 0.3 mol/L and 45 g/mol, and the revolution is 200 r/min, a yield of 77% was reached. Novozym 435 has a high stability. A yield of 60% for ethyl lactate can be obtained with Novozym 435 even after it was used continuously six times.

Key words: lipase, lactic acid, ethanol, esterification, ethyl lactate, double substrates inhibition