催化学报

催化学报 ›› 2006, Vol. 27 ›› Issue (7): 611-614.

• 研究论文 • 上一篇    下一篇

华根霉全细胞脂肪酶催化合成油酸油醇酯

金亮,徐岩,曹光群   

  1. 江南大学工业生物技术教育部重点实验室, 江苏无锡 214036
  • 收稿日期:2006-07-25 出版日期:2006-07-25 发布日期:2006-07-25

Biosynthesis of Oleyl Oleate by Whole-Cell Lipase from Rhizopus chinensis

JIN Liang, XU Yan*, CAO Guangqun   

  1. Key Laboratory of Industrial Biotechnology of the Ministry of Education, Southern Yangtze University, Wuxi 214036, Jiangsu, China
  • Received:2006-07-25 Online:2006-07-25 Published:2006-07-25

PDF

923

被引次数

41

摘要： 比较了10种不同来源的脂肪酶催化油酸与油醇酯化合成油酸油醇酯的能力,其中华根霉Rhizopus chinensis CCTCC M201021全细胞脂肪酶的催化能力最强,其反应转化率可达到90%以上. 酯化反应的最佳油酸/油醇底物摩尔比为1.5, 最佳油酸浓度为0.3 mol/L, 细胞干粉含水量为3.0%～7.5%时对酯化反应最为有利. 以生物相容性指数logP值为指标选择不同的有机溶剂作为有机相进行酯化反应,发现logP值为3.5～4.5的有机溶剂促进酯化效果较好. 全细胞脂肪酶的pH适应范围很广,最佳pH在9.0左右; 最佳反应温度为30 ℃.

关键词: 华根霉, 脂肪酶, 生物催化, 油酸, 油醇, 酯化, 油酸油醇酯

Abstract: The catalytic activity of ten lipases from different sources was compared for the esterification of oleic acid and oleic alcohol to produce oleyl oleate, a new kind of additives used in cosmetic. The whole-cell lipase of Rhizopus chinensis CCTCC M201021 showed the highest conversion (above 90%). Various reaction parameters were optimized to obtain high yield of oleyl oleate. The optimum concentration of oleic acid was 0.3 mol/L, the best organic solvent was n-hexane, and the suitable water content of the dried cells was 3.0%～7.5%. When the molar ratio of oleic acid/oleic alcohol was 1.5, the reaction reached the highest conversion at 30 ℃. The pH range of the whole-cell lipase was wide and the optimal pH value was 9.0.

Key words: Rhizopus chinensis, lipase, biocatalysis, oleic acid, oleic alcohol, esterification, oleyl oleate