催化学报

催化学报 ›› 2006, Vol. 27 ›› Issue (7): 631-635.

• 研究论文 • 上一篇    下一篇

高压CO2条件下粗状假丝酵母催化苯乙酮不对称还原合成α-苯乙醇

应小姣,姚善泾,关怡新   

  1. 浙江大学化学工程与生物工程学系, 浙江杭州 310027
  • 收稿日期:2006-07-25 出版日期:2006-07-25 发布日期:2006-07-25

Asymmetric Reduction of Acetophenone to α-Phenylethanol by Candida valida under Compressed CO2

YING Xiaojiao, YAO Shanjing*, GUAN Yixin   

  1. Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, Zhejiang, China
  • Received:2006-07-25 Online:2006-07-25 Published:2006-07-25

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摘要： 研究了在高压CO2条件下利用粗状假丝酵母Candida valida CICC1444活性细胞催化苯乙酮不对称还原合成(R)-α-苯乙醇. 高压CO2条件可对粗状假丝酵母细胞的活性产生一定的影响,但反应的选择性反而有所提高,证实了高压CO2作为该反应环境是可行的. 考察了底物浓度、细胞用量、反应温度和压力等因素对反应的影响. 结果表明,这些因素对反应产率有很大的影响,在最佳反应条件下,反应底物苯乙酮的转化率和产物α-苯乙醇的产率均可达90%以上,对映体过量值(ee)保持在80%左右. 与常压静置条件下的反应相比,高压CO2条件下粗状假丝酵母催化苯乙酮不对称还原反应的转化率和产率分别提高了20%和40%左右,对映体选择性也提高了5%～10%.

关键词: 高压二氧化碳, 粗状假丝酵母, 全细胞, 生物催化, 苯乙酮, 不对称还原, &alpha, -苯乙醇

Abstract: The asymmetric reduction of acetophenone to optically active α-phenylethanol catalyzed by cells of Candida valida CICC1444 under compressed CO2 without agitation was investigated. The reaction by the unimmobilized whole cells under compressed CO2 was feasible, and the R-enantiomer of α-phenylethanol was excessive. The yield of α-phenylethanol was greatly influenced by operating parameters such as concentration of acetophenone, cell mass, temperature, and pressure. Compared with the reaction at ambient pressure without agitation, the conversion of acetophenone and yield of α-phenylethanol under compressed CO2 were increased by 20% and 40%, respectively, and the enantiomeric excess of (R)-α-phenylethanol was increased by 5%[CD*2/3]10%.

Key words: compressed carbon dioxide, Candida valida CICC1444, whole cell, biocatalysis, acetophenone, asymmetric reduction, α-phenylethanol