催化学报

催化学报 ›› 2008, Vol. 29 ›› Issue (1): 47-51.

• 研究论文 • 上一篇    下一篇

巨大芽胞杆菌环氧水解酶的固定化及其催化性能

贾涛1,许建和1,杨晟2   

  1. 1 华东理工大学生物反应器工程国家重点实验室, 上海 200237; 2 中国科学院上海生命科学研究院植物生理生态研究所, 上海 200032
  • 收稿日期:2008-01-25 出版日期:2008-01-25 发布日期:2011-12-25

Immobilization of Bacillus megaterium Epoxide Hydrolase and Its Catalytic Performance

JIA Tao1, XU Jianhe1*, YANG Sheng2   

  1. 1 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; 2 Shanghai Institute of Plant Physiology and Ecology, The Chinese Academy of Sciences, Shanghai 200232, China
  • Received:2008-01-25 Online:2008-01-25 Published:2011-12-25

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982

被引次数

3

摘要： 考察了多种载体对巨大芽孢杆菌ECU1001环氧水解酶的固定化. 以大孔DEAE-纤维素离子交换树脂为载体时,固定化酶的活力回收达70%. 进一步考察了温度和pH对固定化酶活力的影响,并使用该固定化酶进行了缩水甘油苯基醚对映选择性水解批次反应. 结果表明,在较低的底物浓度下该固定化酶的稳定性较好, 10批反应后仍然剩余72.4%的活力.

关键词: 巨大芽孢杆菌, 环氧水解酶, 固定化, 缩水甘油苯基醚, 水解

Abstract: The immobilization of Bacillus megaterium ECU1001 epoxide hydrolase was investigated. Macro-reticular DEAE-cellulose was selected to immobilize the epoxide hydrolase and the activity retention was about 70%. The effect of temperature and pH on the immobilized epoxide hydrolase was measured. Batch reactions of the hydrolysis of rac-glycidyl phenyl ether were conducted with the immobilized epoxide hydrolase at a low substrate concentration. The residual enzyme activity of 72.4% was achieved after 10 cycles of reaction.

Key words: Bacillus megaterium, epoxide hydrolase, immobilization, glycidyl phenyl ether, hydrolysis