催化学报

催化学报 ›› 2007, Vol. 28 ›› Issue (10): 915-918.

• 研究论文 • 上一篇    下一篇

溴过氧化物酶催化环己烯环氧化反应

于瑶1,2,靳艳1,吴佩春1,张卫1   

  1. 1 中国科学院大连化学物理研究所, 辽宁大连 116023; 2 中国科学院研究生院, 北京 100049
  • 收稿日期:2007-10-25 出版日期:2007-10-25 发布日期:2011-10-28

Epoxidation of Cyclohexene Catalyzed by the Vanadium Bromoperoxidase from Corallina officinalis

YU Yao1,2, JIN Yan1, WU Peichun1, ZHANG Wei1*   

  1. 1 Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023, Liaoning, China; 2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2007-10-25 Online:2007-10-25 Published:2011-10-28

PDF

1296

被引次数

20

摘要： 研究了分离自珊瑚藻(Corallina officinalis)的溴过氧化物酶在水-有机两相体系中催化环己烯环氧化的反应. 考察了氧化剂种类与添加方式、pH、反应温度、酶浓度及KBr浓度等条件对环氧化反应的影响. 最佳反应条件为: 反应温度55 ℃, 在含有50 mmol/L KBr和32 U/ml溴过氧化物酶的pH 6.0的Tris-HCl缓冲液中,每隔10 min补加50 μl的2.29 mol/L H2O2. 在此反应条件下,环氧环己烷的收率达到41.5%, 选择性为82.2%.

关键词: 溴过氧化物酶, 环己烯, 环氧化, 2-环氧环己烷

Abstract: The epoxidation of cyclohexene catalyzed by a vanadium bromoperoxidase from the red algae Corallina officinalis has been investigated. The reaction was performed in a water-organic solvent biphasic system. The effects of oxidants and their loading method, pH, temperature, and the concentration of vanadium bromoperoxidase and KBr on the epoxidation were studied. The optimal conditions were as follows: the reaction temperature was 55 ℃, and 50 μl of 2.29 mol/L H2O2 was added every 10 min to the Tris-HCl buffer at pH 6.0 containing 50 mmol/L of KBr and 32 U/ml of the enzyme (final concentration). Under these conditions, the maximum epoxy cyclohexane yield and selectivity were 41.5% and 82.2%, respectively.

Key words: bromoperoxidase, cyclohexene, epoxidation, epoxy cyclohexane