催化学报

催化学报 ›› 2007, Vol. 28 ›› Issue (7): 662-666.

• 研究论文 • 上一篇    

甲醇驱动的环氧丙烷连续生物合成

辛嘉英1,2,柳眉1,张颍鑫2,夏春谷2,李树本2   

  1. 1 哈尔滨商业大学食品工程学院, 黑龙江哈尔滨 150076; 2 中国科学院兰州化学物理研究所羰基合成和选择氧化国家重点实验室, 甘肃兰州 730000
  • 收稿日期:2007-07-25 出版日期:2007-07-25 发布日期:2011-07-25

Methanol-Driven Continuous Biosynthesis of Epoxypropane by Methanotrophic Bacteria

XIN Jiaying1,2, LIU Mei1, ZHANG Yingxin2, XIA Chungu2, LI Shuben2   

  1. 1 College of Food Engineering, Harbin University of Commerce, Harbin 150076, Heilongjiang, China; 2 State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, The Chinese Academy of Sciences, Lanzhou 730000, Gansu, China
  • Received:2007-07-25 Online:2007-07-25 Published:2011-07-25

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摘要： 采用甲醇蒸气作为碳源对甲基弯菌IMV 3011进行驯化培养,然后逐渐增加液态甲醇的浓度使其适应,得到了能耐受甲醇(φ(MeOH)=1%)的甲基弯菌IMV 3011. 对甲基弯菌IMV 3011进行甲烷-甲醇共培养可得到大量具有甲烷单加氧酶(MMO)活性的细胞. 研究了添加甲醇对甲基弯菌IMV 3011生长和MMO活性的影响,发现甲醇能够促进甲基弯菌IMV 3011的生长. 在批式反应器中,添加甲醇能够提高甲基弯菌IMV 3011的催化环氧化能力,说明甲醇可以作为电子供体通过再生辅酶NADH驱动环氧丙烷合成. 考察了在膜反应器中用细胞悬浮液连续合成环氧丙烷的可行性. 结果表明,通过192 h连续抽提产物环氧丙烷,避免了其对环氧化反应的抑制,流出液中环氧丙烷的浓度仍保持在1.35 mmol/L左右.

关键词: 甲醇驱动, 甲烷氧化细菌, 辅酶, 尼克酰胺腺嘌呤二核甘酸(NADH), 生物合成, 丙烯, 环氧丙烷

Abstract: Methylosinus trichosporium IMV 3011 was cultivated on methanol vapor as carbon source. The methanol vapor-grown culture was adapted to grow on liquid methanol with its gradually increasing concentrations from 0.01% to 1%(volume fraction). After adaptation to high methanol levels, M. trichosporium IMV 3011 was cultured on methanol-methane to obtain a large quantity of cells with methane monooxygenase (MMO) activity. The effect of methanol on the growth of M. trichosporium IMV 3011 and MMO activity was studied. The growth of methanotrophic bacteria was significantly enhanced in comparison to the absence of methanol. In the batchwise epoxidation, the epoxidation capacity of M. trichosporium IMV 3011 was enhanced when methanol was provided, indicating that the methanol could act as an electron donator to drive the synthesis of epoxypropane. Also, the possibility of methanol-driven continuous synthesis of epoxypropane by M. trichosporium IMV 3011 was explored in a membrane reactor. At the most suitable liquid flow velocity of6 ml/h, the epoxypropane concentration in eluate was 2.19 mmol/L and the residual methanol concentration was 1.08 mmol/L. The product inhibition was overcome owing to the continuous removal of epoxypropane. In this process, the reactor was operated continuously for 192 h, and the epoxypropane concentration in eluate was kept to about 1.35 mmol/L. Direct counts and plate counts for M. trichosporium IMV 3011 showed that the bacteria could survive in the process of methanol-driven epoxypropane synthesis.

Key words: methanol-driving, methanotrophic bacteria, coenzyme, nicotinamide adenine dinucleotide (NADH), biosynthesis, propene, epoxypropane