催化学报

催化学报 ›› 2008, Vol. 29 ›› Issue (1): 41-46.

• 研究论文 • 上一篇    下一篇

华根霉全细胞脂肪酶催化合成生物柴油

贺芹,徐岩,滕云,王栋   

  1. 江南大学生物工程学院教育部工业生物技术重点实验室, 江苏无锡 214122
  • 收稿日期:2008-01-25 出版日期:2008-01-25 发布日期:2011-12-25

Biodiesel Production Catalyzed by Whole-Cell Lipase from Rhizopus chinensis

HE Qin, XU Yan*, TENG Yun, WANG Dong   

  1. Key Laboratory of Industrial Biotechnology of the Ministry of Education, Jiangnan University, Wuxi 214122, Jiangsu, China
  • Received:2008-01-25 Online:2008-01-25 Published:2011-12-25

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1437

被引次数

112

摘要： 比较了5种不同商品化脂肪酶和自制的华根霉CCTCC M201021全细胞脂肪酶(RCL)催化油脂合成生物柴油的转化效果,结果表明, RCL能有效应用于无溶剂体系催化合成生物柴油. 在无溶剂体系中对该酶催化生物柴油的转酯化反应工艺进行优化,考察了甲醇用量、体系含水量、酶的添加量和反应温度对生物柴油收率的影响,使生物柴油最终收率大于86.0%. 在有机溶剂体系中选择不同有机溶剂作为助溶剂进行转酯化反应,发现log P值在4.0～4.5的有机溶剂具有较好的转化效果. 其中以正庚烷为助溶剂的转酯化反应具有最高的生物柴油收率86.7%. 在无溶剂体系中RCL催化转化油酸和模拟高酸价油脂合成脂肪酸甲酯的研究表明,该酶具有很好的催化合成生物柴油的潜力.

关键词: 华根霉, 全细胞脂肪酶, 转酯化反应, 生物柴油, 脂肪酸甲酯, 无溶剂体系, 高酸价油脂

Abstract: Compared with five commercial lipases, the whole-cell lipase of Rhizopus chinensis CCTCC M201021 (RCL) showed high catalytic ability in the transesterification of soybean oil to fatty acid methyl ester (biodiesel) in the solvent-free system. The effect of reaction parameters including methanol content, water content, lipase amount, and reaction temperature on transesterification was investigated. The biodiesel yield was more than 86.0% in the solvent-free system under the optimal reaction conditions. The effect of different organic solvents on the transesterification indicated that the best one was n-heptane with higher log P values(4.0-4.5). The whole-cell lipase was also proved to be an efficient and potential biocatalyst for biodiesel production from oleic acid and simulant high acid value oil in the solvent-free system.

Key words: Rhizopus chinensis, whole-cell lipase, transesterification, biodiesel, fatty acid methyl ester, solvent-free system, high acid value oil