催化学报 ›› 2014, Vol. 35 ›› Issue (4): 524-531.DOI: 10.1016/S1872-2067(14)60017-6

• 研究论文 • 上一篇    下一篇

氮杂大环铈(Ⅲ)配合物与pUC19 DNA的相互作用

王敏, 俞兰, 李方珍, 谢家庆   

  1. 重庆理工大学化学化工学院, 重庆400054
  • 收稿日期:2013-11-18 修回日期:2013-12-30 出版日期:2014-03-20 发布日期:2014-03-21
  • 通讯作者: 谢家庆
  • 基金资助:

    国家自然科学基金项目(21173274);自贡科技局重点计划项目(2013X03);四川教育厅项目(13ZA0126).

The interactions between an aza-cyclic cerium (Ⅲ) complex and pUC19 DNA

Min Wang, Lan Yu, Fangzhen Li, Jiaqing Xie   

  1. College of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing 400054, China
  • Received:2013-11-18 Revised:2013-12-30 Online:2014-03-20 Published:2014-03-21
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (21173274), Major Science and Technology Project of Zigong City (2013X03), and the Educational Department of Sichuan Government (13ZA0126).

摘要:

以一种1,4,7,10,13,16,19,22,25,28,31-十一氮杂2,5,8,11,14,17,20,23,26,29,32-十一酰基-环三十三烷的衍生物(CYC)为配体,在溶液中和Ce(Ⅲ,M)离子形成氮杂大环铈配合物(M-CYC)作为仿生催化剂,通过紫外光谱法、荧光光谱法和凝胶电泳法研究了M-CYC配合物与DNA的相互作用. 结果表明,M-CYC与小牛胸腺DNA(CT DNA)以嵌插方式相互作用,在pH = 8.16的缓冲体系中,其结合常数Kb = 2.0×104 L/mol. 凝胶电泳结果表明,当自由基捕捉剂存在时,M-CYC可将超螺旋pUC19 DNA切割为缺刻型DNA,其切割方式为水解切割.

关键词: 氮杂大环, 铈(Ⅲ), 配合物, DNA, 切割

Abstract:

A new biomimetic catalyst (M-CYC) is formed from the combination of CYC (a derivative of 1,4,7,10,13,16,19,22,25,28,31-undecaaza-cyclotritriacontane-2,5,8,11,14,17,20,23,26,29,32-undeca acyl) and Ce(Ⅲ, M) in aqueous solution. The interaction between this complex and DNA was studied using electronic absorption spectroscopy, fluorescence spectroscopy, and gel electrophoresis. The results indicated that M-CYC and calf thymus DNA (pUC19 DNA) interacted via intercalation. The apparent binding constant of the complex with DNA was determined to be 2.0×104 L/mol at pH 8.16. Based on gel electrophoresis analysis, M-CYC was shown to nick the circular plasmid pUC19 DNA in the presence of a free radical scavenger. The nicking of DNA serves as a model of hydrolyzed cleavage.

Key words: Aza-cycle, Cerium, Complex, DNA, Cleavage