催化学报

催化学报 ›› 2014, Vol. 35 ›› Issue (5): 763-769.DOI: 10.1016/S1872-2067(14)60040-1

• 研究论文 • 上一篇    下一篇

超临界二氧化碳偶合超声预处理强化玉米秸秆酶水解

银建中, 郝刘丹, 喻文, 王恩俊, 赵孟姣, 徐琴琴, 刘一凡   

  1. 大连理工大学化工机械学院, 精细化工国家重点实验室, 辽宁大连116024
  • 收稿日期:2013-11-14 修回日期:2014-01-20 出版日期:2014-04-18 发布日期:2014-04-24
  • 通讯作者: 银建中
  • 基金资助:

    国家自然科学基金（20976026，20976028，21376045）；高等学校博士学科点专项科研基金（20120041110022）；辽宁省自然科学基金（20102030，2013220148）.

Enzymatic hydrolysis enhancement of corn lignocellulose by supercritical CO2 combined with ultrasound pretreatment

Jianzhong Yin, Liudan Hao, Wen Yu, Enjun Wang, Mengjiao Zhao, Qinqin Xu, Yifan Liu   

  1. State Key Laboratory of Fine Chemicals, School of Chemical Machinery, Dalian University of Technology, Dalian 116024, Liaoning, China
  • Received:2013-11-14 Revised:2014-01-20 Online:2014-04-18 Published:2014-04-24
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (20976026, 20976028, 21376045), the Specialized Research Fund for the Doctoral Program of Higher Education (20120041110022), and the Natural Science Foundation of Liaoning Province (20102030, 2013220148).

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603

被引次数

7

摘要：

提出了一个木质纤维素生物质预处理的全绿色加工过程. 以玉米秸秆和玉米芯为原料，以超临界CO2和超声偶合法对木质纤维素进行预处理. 超临界CO2预处理条件为： 压力15-25 MPa，温度120-170 ℃，含水量50%，反应时间0.5-4 h. 超声场功率600 W，温度80 ℃，作用时间2-8 h. 用纤维素酶水解反应获得的还原糖总量来评价预处理效果. 结果表明，单纯超临界CO2和超临界CO2偶合超声预处理都能够提高生物质水解反应还原糖产量. 对于玉米芯，超临界CO2预处理（170 ℃，20 MPa，30 min）后，还原糖产率为62%（未预处理的为12%）. 对于玉米秸秆（170 ℃，20 MPa，2.5 h），还原糖产率为46.4%. 对于玉米芯，超临界CO2偶合超声预处理（600 W，80 ℃下超声处理6 h，然后用170 ℃，20 MPa超临界CO2预处理30 min）后，还原糖产率为87%. 对于玉米秸秆，超临界CO2偶合超声预处理（600 W，80 ℃下超声处理8 h，然后用170 ℃，20 MPa超临界CO2预处理1 h）后，还原糖产率为25.5%. 与未处理生物质相比，X射线衍射结果表明玉米秸秆和玉米芯在超临界CO2和超声预处理后其结晶度没有明显变化. 扫描电镜分析则发现木质纤维素的表面积显著增加.

关键词: 木质纤维素, 预处理, 超临界CO2, 超声场, 酶水解

Abstract:

Supercritical carbon dioxide (scCO2) combined with an ultrasound method using 15-25 MPa and the temperature range of 120-170 ℃ for 0.5-4 h was proposed as a pretreatment for corn cob and corn stalk with moisture contents of 50%. The results showed that both scCO2 pretreatment and scCO2 combined with ultrasound pretreatment could improve the total reducing sugar yields of the two materials. Enzymatic hydrolysis of both corn cob and corn stalk sugar using scCO2 pretreatment was increased by 50% and 29.8% compared with the control samples, respectively, while they were increased by 75% and 13.4%, respectively, using the scCO2 combined with ultrasound pretreatment under our experimental conditions. Following treatment from the two pretreatment processes, X-ray diffraction results indicated no significant change in crystallinity of the two materials. However, scanning electron microscopy images demonstrated that the microscopic structure of lignocellulose changed considerably and the surface area of lignocellulose increased significantly.

Key words: Lignocellulose, Pretreatment, Supercritical carbon dioxide, Ultrasound, Enzymatic hydrolysis