基于光合作用的生物分子马达与光系统II的共组装

摘要/Abstract

摘要： 近年来分子组装技术已成为化学、物理、生物和材料等交叉学科的前沿.生物分子马达是存在于几乎所有生物系统中的天然分子机器.这些生物分子机器在细胞生命活动中参与胞内运输、能量转换和肌肉收缩等一系列重要的生命过程，起着举足轻重的作用.旋转分子马达ATP合酶是目前研究最多的生物分子机器之一.开展ATP合酶的体外重组，不仅有助于理解其在生物过程中的工作机制，而且也有助于推动生物分子马达基生物传感以及合成分子机器的发展.本综述将主要介绍本课题组近期如何设计和构建基于ATP合酶的活性仿生体系的研究进展.受植物中光合磷酸化作用的启发，我们在早期的ATP合酶与层层组装的类细胞结构体共组装的基础上，将光系统Ⅱ（PSⅡ）引入组装体中，构筑了一系列具有光响应功能的ATP合成体系，成功模拟了自然界中光合作用光能与生物能的转化过程.进一步，在天然叶绿体中引入功能组分，制备了人工杂化叶绿体，显著提高了杂化体系的光磷酸化效率.这些复合组装体既成功地模拟了自然界中光合作用的化学反应过程，也为提升光能的有效利用提供了新途径.

关键词: 生物分子机器, ATP合酶, 光系统II, 光酸分子, 量子点, 层层组装, 微球, 多层膜

Abstract: Molecular assembly is an important frontier research in chemistry, physics, biology and material. Biomolecular motors are natural molecular machines that exist in nearly all the biological systems. These biomolecular motors play crucial roles in life activities and participate in a series of important life activities such as intracellular transport, energy transfer and muscle contraction and so on. Rotating molecular motor ATP synthase is one of the most studied biomolecular machines. The recombination of ATP synthase in vitro not only helps to better understand its working mechanism in biological processes, but also promotes the development of biomolecular motor-based devices and synthetic molecular motors. In this paper, we mainly introduce our latest progress on the design and construction of ATP synthase-based biomimetic system. Inspired by photophosphorylation in plants, we fabricated a series of light-responsive ATP synthesis systems by co-assembling ATP synthase and photosystem Ⅱ (PSⅡ) within layer-by-layer assembled cell-like structures. These systems effectively simulated the transformation process from light energy to biological energy in natural photosynthesis. Besides, we also constructed artificial hybrid chloroplasts by introducing functional components into natural chloroplasts. Compared to natural chloroplasts, these hybrid chloroplasts showed remarkable improvement of photophosphorylation efficiency for ATP synthesis. These composite assemblies well simulate the chemical reaction process of photosynthesis in nature, and provide a new way for the effective utilization of light.

Key words: biomolecular machine, ATP synthase, photosystem II, photoacid generator, quantum dot, Layer-by-Layer, microsphere, multilayer

贾怡, 许有前, 冯熙云, 李悦, 李广乐, 李峻柏. 基于光合作用的生物分子马达与光系统II的共组装[J]. 催化学报, 2019, 40(s1): 143-148.

JIA Yi, XU Youqian, FENG Xiyun, LI Yue, LI Guangle, LI Junbai. Co-assembly of Molecular Motors ATP synthase and Photosystem II based Photosynthesis[J]. Chinese Journal of Catalysis, 2019, 40(s1): 143-148.

×
扫码分享

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://www.cjcatal.com/CN/

https://www.cjcatal.com/CN/Y2019/V40/Is1/143

参考文献

1 Balzani V, Gómez-López M, Stoddart J F. Acc Chem Res, 1998, 31:405
2 Sauvage J P. Angew Chem Int Ed, 2017, 56:11080
3 Erbas-Cakmak S, Leigh D A, McTernan C T, Nussbaumer A L. Chem Rev, 2015, 115:10081
4 Guix M, Mayorga-Martinez C C, Merkoçi A. Chem Rev, 2014, 114:6285
5 Boyer P D. Annu Rev Biochem, 1997, 66:717
6 Boyer P D. Angew Chem Int Ed, 1998, 37:2297
7 Ueno H, Suzuki T, Kinosita K, Yoshida M. Proc Natl Acad Sci USA, 2005, 102:1333
8 Choi H J, Germain J, Montemagno C D. Nanotechnology, 2006, 17:1825
9 Pitard B, Richard P, Dunach M, Girault G, Rigaud J L. Eur J Biochem, 1996, 235:769
10 Choi H J, Montemagno C D. Nanotechnology, 2006, 17:2198
11 Choi H J, Montemagno C D. Nano Lett, 2005, 5:2538
12 Walde P, Ichikawa S. Biomol Eng, 2001, 18:143
13 Duan L, He Q, Wang K, Yan X, Cui Y, Moewald H, Li J. Angew Chem Int Ed, 2007, 46:6996
14 Duan L, Qi W, Yan X, He Q, Cui Y, Wang K, Li D, Li J. J Phys Chem B, 2008, 113:395
15 Qi W, Duan L, Wang K, Yan X, Cui Y, He Q, Li J. Adv Mater, 2008, 20:601
16 Luo T J M, Soong R, Lan E, Dunn B, Montemagno C. Nat Mater, 2005, 4:220
17 Steinberg-Yfrach G, Rigaud J L, Durantini E N, Moore A L, Gust D, Moore T A. Nature, 1998, 392:479
18 Wang W Y, Chen J, Li C, Tian W M. Nat Commun, 2014, 5:4647
19 Wang W Y, Wang H, Zhu Q J, Qin W, Han G Y, Shen J R, Zong X, Li C. Angew Chem Int Ed, 2016, 55:9229
20 Feng X Y, Jia Y, Cai P, Fei J, Li J B. ACS Nano, 2016, 10:556
21 Li G L, Fei J B, Xu Y Q, Li Y, Li J B. Adv Funct Mater, 2018, 28:1706557
22 Li Y, Fei J B, Li G L, Xie H T, Yang Y, Li J L, Xu Y Q, Sun B B, Xia J R, Fu X Q, Li J B. ACS Nano, 2018, 12:1455
23 Xu Y Q, Fei J B, Li G L, Yuan T T, Li Y, Wang C L, Li X B, Li J B. Angew Chem Int Ed, 2017, 56:12903
24 Xu Y Q, Fei J B, Li G L, Yuan T T, Xu X, Wang C L, Li J B. Angew Chem Int Ed, 2018, 57:6532
25 Shi Z, Peng P, Strohecker D, Liao Y. J Am Chem Soc, 2011, 133:14699
26 Xu Y Q, Fei J B, Li G L, Yuan T T, Li J B. ACS Nano, 2017, 11:10175
27 Brown K A, Harris D F, Wilker M B, Rasmussen A, Khadka N, Hamby H, Keable S, Dukovic G, Peters J W, Seefeldt L C, King P W. Science, 2016, 352:448
28 Chen C W, Wu D Y, Chan Y C, Lin C C, Chung P H, Hsiao M, Liu R S. J Phys Chem C, 2015, 119:2852

[1]	刘德法, 孙彬, 白硕杰, 高婷婷, 周国伟. 双助催化剂Ag/Ti₃C₂/TiO₂分层花状微球用于增强光催化产氢活性[J]. 催化学报, 2023, 50(7): 273-283.
[2]	蒋洁, 王国宏, 邵琰池, 王娟, 周双, 苏耀荣. 增强制氢性能的ZnO@ZnS空心微球S型异质结光催化剂[J]. 催化学报, 2022, 43(2): 329-338.
[3]	刘玉华, 张伟, 郑伟涛. MXene量子点的表面化学: 受病毒机制启发的催化微型实验室[J]. 催化学报, 2022, 43(11): 2913-2935.
[4]	关晨, 岳晓阳, 范佳杰, 向全军. MXene量子点(Ti₃C₂): 性质、合成及其在能源领域的应用[J]. 催化学报, 2022, 43(10): 2484-2499.
[5]	荣佳悦, 王珍珍, 吕嘉奇, 范明, 种瑞峰, 常志显. Ni(OH)₂量子点助催化剂修饰α-Fe₂O₃光阳极增强光电分解水性能[J]. 催化学报, 2021, 42(11): 1999-2009.
[6]	高荣荣, 程蓓, 范佳杰, 余家国, WingkeiHo. 锌镉硫量子点增强的光催化产氢活性[J]. 催化学报, 2021, 42(1): 15-24.
[7]	何烨, 李解元, 李康璐, 孙明禄, 袁潮苇, 陈瑞敏, 盛剑平, 冷庚, 董帆. Bi量子点修饰的C掺杂二维BiOCl纳米片:增强的可见光光催化活性和反应路径[J]. 催化学报, 2020, 41(9): 1430-1438.
[8]	李凯, 季梦夏, 陈蓉, 姜琪, 夏杰祥, 李华明. 氮磷共掺杂石墨烯量子点/Bi₅O₇I复合材料的构筑及增强的光催化降解性能[J]. 催化学报, 2020, 41(8): 1230-1239.
[9]	徐浩, 田文文, 徐丽萍, 金鑫, 薛腾, 陈丽, 何鸣元, 吴鹏. TS-2微球的形成机理、后处理修饰和催化应用[J]. 催化学报, 2020, 41(7): 1109-1117.
[10]	徐婧, 黄金, 王周平, 朱永法. 氧化石墨烯量子点修饰氧掺杂多孔g-C₃N₄在光催化降解和抗菌中的应用[J]. 催化学报, 2020, 41(3): 474-484.
[11]	冯晓珊, 郑颖滨, 林代峰, 吴恩惠, 罗永晋, 游钰锋, 薛珲, 钱庆荣, 陈庆华. 用于氯乙烯废气高效催化氧化的Ce-Cu-W-O微球的新颖合成[J]. 催化学报, 2020, 41(12): 1864-1872.
[12]	马贺, 王长华, 李松美, 张昕彤, 刘益春. TiO₂(B)多孔微球高湿光热催化降解NO_x性能[J]. 催化学报, 2020, 41(10): 1622-1632.
[13]	戚克振, 吕文秀, Iltaf Khan, 刘书源. 化学镀法制备CoP量子点修饰g-C₃N₄用于光催化产氢[J]. 催化学报, 2020, 41(1): 114-121.
[14]	秦海燕, 彭笑刚. 光谱学与新材料:量子点光谱学的关键科学问题[J]. 催化学报, 2019, 40(s1): 98-103.
[15]	肖颖冠, 孙孝东, 李霖昱, 陈娟荣, 赵仕东, 蒋采国, 杨露瑶, 程黎, 曹顺生. 碳-氮共改性中空二氧化钛光催化剂的同步合成及其高效的光催化行为和循环稳定性研究[J]. 催化学报, 2019, 40(5): 765-775.